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. 2020 Oct 11;12(10):2917. doi: 10.3390/cancers12102917

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Functional classification of EVs proteins synthesized by hypoxic A549 lung cancer cells. (A) Bar chart showing distribution of membrane proteins according to sub-cellular localization (as assigned by the PANTHER functional classification system. (B) Western blots (left) confirming expression levels of selected membrane-associated proteins by A549 cells when subjected to hypoxia (Hx) or normal culture conditions (Nx). Experiment was done with 3 biological replicates. Ponceau S staining as protein loading control (center) and the analysis was quantified by densitometry (right). (C) Bar chart showing distribution of membrane proteins according to functional class. Detailed information about Western blot can be found at Figure S1.