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. 2020 Oct 11;12(10):2917. doi: 10.3390/cancers12102917

Figure 4.

Figure 4

EVs released by oxygen-starved tumours promote EMT in recipient A549 lung cancer cells. Bar chart showing signaling pathways triggered by EVs derived from A549 lung cancer cells in normoxic cultures (Nx) or hypoxic conditions (Hx) based on the PANTHER functional classification system (A). Representative fluorescent images of EV-treated A549 lung cancer and EV-treated H1299 lung cancer showed actin rearrangement as revealed by staining with phalloidin (green). Magnification = 100× (B). Western blots of E-cadherin and N-cadherin expression levels in A549 cells and H1299 cells when subjected to hypoxia or normoxia derived EVs treatment; experiment was repeated with 3 biological replicates. Error bars indicate S.E.M of three biological replicates (C). Gene expression levels of characteristic EMT markers, Snail, slug and E-cadherin in A549 cells (D) and H1299 cells (E) after EV treatment. Error bars indicate S.E.M of three biological replicates. Detailed information about Western blot can be found at Figure S1.