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. 2020 Sep 25;9(10):2170. doi: 10.3390/cells9102170

Figure 5.

Figure 5

Expression of PDE4-D and intracellular levels of cAMP. (A) Western blot analysis of PDE4-D; α-tubulin was used as the loading control, N ≥ 5. (B) Densitometric evaluation of the PDE4-D band intensity, N ≥ 5. (C) Total basal PDE activity of Epac1-KO and the corresponding WT control cells, N = 4, n ≥ 8. (D) The basal intracellular cAMP levels in the WT and Epac1-KO endothelial cells; N ≥ 10. (E) The cAMP levels of the WT and KO cells subjected to F/R, N ≥ 5; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; “n.s.” denotes non-significant differences between the analyzed groups.