Figure 4.

(A) Confirmation of correlation of optical density measurement with the MTT method and plaque counting. Vero cells (10,000 cells) were inoculated on a 96-microwell plate and incubated overnight at 37 °C. HSV-1 solution at the indicated dilution fold was then added. After incubation for three days, viable cell number (absorbance at 595 nm) was determined by the MTT method, and plaque formation was counted by light microscopy. (B) Effect of different MOI on the anti-HSV activity of ACV. Each value represented as mean ± S.D. was determined (n = 3). Significant difference between MOI = 0.01 and MOI = 0.1 (p < 0.05).