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. 2020 Oct 30;40(10):BSR20201111. doi: 10.1042/BSR20201111

Figure 3. Restoration of let-7g-5p decreased the TMZ resistance and malignant phenotypes of GSCs.

Figure 3

(A) The expression level of let-7g-5p in the serum samples of normal volunteers and GBM patients were calculated by qRT-PCR. (B) The expression level of let-7g-5p in GSCs and non-GSCs were analyzed using qRT-PCR. (C) qRT-PCR was implied to detect the level of let-7g-5p after treating with TMZ alone or co-treating with TMZ and let-7g-5p mimics. (D) Cell viability was detected by CCK-8 assay to analysis the effect of let-7g-5p up-regulation on TMZ resistance of GSCs. (E,F) The migration and invasion of GSCs after treating with TMZ alone or co-treating with TMZ and let-7g-5p mimics were measured by transwell. (G) Western blot assay was used to evaluate the protein levels of E-cadherin and N-cadherin in GSCs after treating TMZ alone or co-treating TMZ+let-7g-5p mimics. Data were presented as mean ± SD; *P<0.5, **P<0.01, ***P<0.001.