Table 2.

Parameters of DNA extracted from opium poppy native seeds, ground seeds, and pollen grains by different extraction procedures and commercial kits.

P. somniferum L. Matrix	DNA Extraction Method	DNA Yield (μg)	DNA Concentration (ng/μL)	A260/280	A260/230	Target Locus
						psSSR69	Tubulin II
Native seeds	Dellaporta et al. [24]	82.76 *	413.78 *	1.66 *	0.87 *	+	+
		90.68	453.39	1.62	0.84	+	+
	Dellaporta et al. [24] with CTAB	53.32 *	266.62 *	1.62 *	0.83 *	+	+
		73.19	365.94	1.55	0.81	+	+
	Bayer BioScience N.V. [25]	115.13	575.63	1.81	1.84	+	+
	Murray, Thompson [27]	289.84	483.07	2.10	2.37	+	+
	Monsanto Company [26]	15.52	62.06	1.77	2.21	+	+
	Sagwan et al. [21]	108.21	1082.14	1.96	2.51	+	+
	Newly developed protocol	248.30	703.85	1.91	2.19	+	+
	DNeasy® Plant Maxi Kit	108.83	145.11	1.96	2.18	+	+
	Plant DNAzol® Reagent	137.40	114.50	1.54	0.28	-	-
	QIAamp DNA Stool Mini Kit	172.68	863.39	2.11	2.32	+	+
	PowerSoil DNA Isolation Kit	1.40	13.97	1.75	0.72	+	-
Ground seeds	Bayer BioScience N.V. [25] (1)	18.43	92.17	1.79	1.12	+	+
	Bayer BioScience N.V. [25] (2)	110.01	550.06	1.90	2.21	+	+
	Monsanto Company [26]	1.35	5.38	1.76	1.86	+	+
	DNeasy® Plant Maxi Kit	6.28	35.88	1.53	0.64	+	+
	QIAamp DNA Stool Mini Kit	37.96	189.79	2.13	2.25	+	+
	Newly modified protocol	175.11	437.77	1.90	2.19	+	+
Pollen grains	Newly developed protocol	210.7	2107.0	1.95	2.17	+	+
	DNeasy® Plant Mini Kit	2.22	22.2	1.92	1.1	+	+
	QIAamp DNA Stool Mini Kit	3.24	16.2	2.15	1.47	+	+

*—Homogenization of seeds with liquid nitrogen; Bayer BioScience N.V. [25] (1)—0.2 g of seeds extracted in volume of extraction buffer for 0.2 g seeds; Bayer BioScience N.V. [25] (2)—DNA extracted from 0.2 g of seeds extracted in volume of extraction buffer for 0.5 g of seeds.