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. 2020 Oct 3;10(10):1799. doi: 10.3390/ani10101799

Figure 3.

Figure 3

On-station trial for the evaluation of in vivo anthelmintic properties of plants. When a cafeteria trial of two plant species are performed, it is recommended that the candidate for nutraceutical properties contains a known quantity of a plant secondary compound while the other plant does not. (a,b) represent the first and second experimental days, respectively. For the establishment of control groups, a polymer (polyethylene glycol) could be used to neutralize condensed tannins and other polyphenols. Activated charcoal could be used to neutralize some terpenes. Containers in which the plants are offered must be equidistant and changed on a daily basis to avoid associate learning. Some researchers choose to hang experimental plants to simulate browsing ingestive behaviors. Such considerations should be in function of the facilities where trials are conducted.