Figure 4. The introduction of UCH-L1 inhibitor LDN-57444 blocked spindle body formation during mouse oocyte maturation.

Mouse oocytes were matured with DMSO or LDN-57444 (20 µM). After 18 h, oocytes were fixed, incubated with FITC-conjugated α-tubulin antibody, stained with Hoechst 33342 solution and analyzed by confocal microscopy. Green, α-tubulin; blue, Hoechst 33342 for DNA staining. **P<0.01.