Table 3.
Summary of the experimental conditions employed for fish collagen isolation using pepsin aided AcOH extraction.
| Source of Collagen | Extraction Solvent | Extraction Conditions | Yield (Y) | Ref |
|---|---|---|---|---|
| Thornback ray skin | 0.2 M AcOH | T = 4 °C Time = 18 h S/L = 1/10 5 g of pepsin/g of skin |
Y = 30.16% | [89] |
| Scales of seabass | 0.5 M AcOH | T = 4 °C Time = 48 h S/L = 1/10 20 g of pepsin/g of skin |
Y = 1.06% | [82] |
| Jellyfish | 0.6 M AcOH | T = 4 °C Time = 72 h S/L = 1/10 1% pepsin |
Y = 0.28% | [90] |
| Skins of catla and rohu fish | 0.5 M AcOH | T = 4 °C Time = 48 h S/L = 1/60 |
Y = 69% (catla) Y = 65% (rohu) |
[83] |
| Skin of giant croaker | 0.5 M AcOH | Pepsin concentration = 800–2400U/g S/L = 1:45–1:65 Time = 6–10 h pH = 1 to 4 T = 4 °C |
Y = 84.85% | [87] |
| By-products of bigeye tuna | 0.5 M AcOH | T = 4 °C Time = 48 h S/L = 1/40 0,2 g of pepsin/g of material |
Y of bone = 2,6% Y of scale = 4,6% Y of skin = 16,7% |
[64] |
| Cod swim bladders | 0.5 M AcOH | T = 25 °C Time = 3 days S/L = 1/10 10% pepsin |
Y = 11.53% | [91] |
| Catfish skin | HCl | pH = 2.4 S/L = 1/5 to 1/20 Pepsin concentration = 0.118 to 23.6 KU/g T = 4 °C |
Y = 59.03% | [78] |
| Nilem fish skin | 0.5, 0.7, and 0.9 M AcOH | Pepsin concentration = 0.5, 1, and 1.5% T = 4 °C |
Y = 4.25–6.18% | [88] |
| Silver carp (Hypophthalmichthys molitrix) scales | 0.5 M AcOH | T = 4 °C S/L = 1/10–1/50 Time = 10–60 h 1–5% Pepsin |
Y = the maximum yield 12.06% | [92] |
| Lophius litulon skin | 0.5 M AcOH | T = 4 °C 1–6% Pepsin |
Y = not evaluated | [93] |
| Golden pompano (Trachinotus blochii) Skin and Bone | 0.5 M AcOH | T = 4 °C S/L = 1/40 Time = 48 h |
Y of skin = 21.81% Y of bone = 1.25% |
[94] |
| Tilapia skin | 0.5 M AcOH | Time = 48 h T = 4 °C 0.5% Pepsin |
Y = not evaluated | [95] |
| Sardinella longiceps (oil Sardine) Scale | 0.5 M AcOH | Time = 4 days T = 4 °C S/L = 1/15 Pepsin = 40 unit/g of residue |
Y = 3% | [85] |