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. 2020 Oct 2;12(10):2850. doi: 10.3390/cancers12102850

Figure 3.

Figure 3

Effect of PDIA1 inhibition on adhesion of breast cancer cells to the endothelium, collagen type I and fibronectin and their transendothelial migration across hLMVEC monolayer. Effect of bepristat 2a on adhesion of MCF-7 (A,E) and MDA-MB-231 (B,F) cells to hLMVEC, collagen type I and fibronectin, respectively, and MCF-7 (C,G) and MDA-MB-231 (D,H) cells transduced with lentiviral vectors carrying short hairpin RNA (shRNA) against PDIA1 or negative sequence after selection with puromycin in regarding to wild type cell line. Transendothelial migration of MCF-7 (I) and MDA-MB-231 (J) cells treated with bepristat 2a and MCF-7 (K) and MDA-MB-231 (L) sublines with silenced PDIA1 expression across hLMVEC monolayers. Data represent mean ± SD of three independent experiments. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test or nonparametric Kruskal–Wallis followed by Dunn’s multiple comparisons test (* p = 0.05, ** p = 0.01, *** p = 0.001, **** p = 0.0001).