Table 1.
Relative content of protein disulphide isomerases (PDIs) in hLMVEC, MCF-7 and MDA-MB-231 cell lines.
| PDI Isoform | hLMVEC [Mean ± SD] | MCF-7 [Mean ± SD] | MDA-MB-231 [Mean ± SD] |
|---|---|---|---|
| PDIA1 | 10.297 ± 1.374 | 8.987 ± 2.895 | 7.220 ± 0.907 |
| PDIA3 | 7.313 ± 0.918 | 7.920 ± 4.092 | 5.063 ± 0.903 |
| PDIA4 | 4.020 ± 0.000 | 4.387 ± 2.620 | 2. 943 ± 1.101 |
| PDIA5 | 0.233 ± 0.085 | 0.235 ± 0.120 | not detected |
| PDIA6 | 3.503 ± 0.231 | 2.807 ± 0.512 | 1.833 ± 0.006 |
| PDIA9 | 2.160 ± 0.000 | 2.630 ± 1.250 | 2.190 ± 0.460 |
| PDIA10 | 0.660 ± 0.087 | 1.380 ± 0.314 | 0.663 ± 0.179 |
| PDIA11 | 0.420 ± 0.104 | 0.850 ± 0.678 | 0.567 ± 0.319 |
| PDIA12 | 0.130 ± 0.104 | 0.130 ± 0.000 | 0.130 ± 0.000 |
| PDIA13 | 0.177 ± 0.095 | not detected | 0.130 ± 0.071 |
| PDIA14 | 0.110 ± 0.000 | 0.240 ± 0.000 | not detected |
| PDIA15 | 3.443 ± 1.564 | 1.670 ± 0.962 | 1.783 ± 0.365 |
| PDIA16 | 1.217 ± 0.266 | 0.440 ± 0.173 | 0.540 ± 0.000 |
| PDIA17 | not detected | 6.117 ± 1.58 | 0.230 ± 0.000 |
| PDIA18 | not detected | 1.640 ± 0.896 | not detected |
| PDIA19 | 0.380 ± 0.104 | 0.260 ± 0.000 | 0.100 ± 0.000 |
Semi-quantitative data (emPAI) for each detected PDI isoform in lysates from human lung microvascular endothelial cells (hLMVEC) and breast cancer MCF-7 and MDA-MB-231 cell lines. Data represent the means ± SD from three consecutive passages. emPAI was calculated based on the number of observed peptides per proteins normalized by the theoretical number of peptides subtracted from LC-MS/MS data using MascotTM (Matrix Sciences, London, UK).