Table 2.
Relative content of PDIs (emPAI) in MCF-7 and MDA-MB-231 cell lines after PDIA1 silencing.
| PDI Isoform | MCF-7 Cells | MDA-MB-231 Cells | ||||||
|---|---|---|---|---|---|---|---|---|
| Wild Type | shN | shPDIA1-1 | shPDIA1-3 | Wild Type | shN | shPDIA1-1 | shPDIA1-3 | |
| PDIA1 | 9.032 ± 0.846 | 8.845 ± 1.853 | 0.163 ± 0.085 | 2.043 ± 0.611 | 5.002 ± 0.508 | 2.000 ± 1.185 | 4.348 ± 2.359 | 1.145 ± 0.382 |
| PDIA3 | 7.454 ± 1.309 | 7.700 ± 2.079 | 7.388 ± 1.078 | 7.597 ± 1.594 | 5.366 ± 1.523 | 6.653 ± 1.631 | 5.418 ± 0.979 | 7.565 ± 1.292 |
| PDIA4 | 5.448 ± 0.879 | 5.190 ± 0.846 | 5.630 ± 0.664 | 2.707 ± 0.320 | 3.698 ± 0.947 | 4.035 ± 0.424 | 4.270 ± 0.757 | 3.508 ± 0.622 |
| PDIA5 | 0.070 ± 0.000 | 0.150 ± 0.080 | 0.150 ± 0.080 | 0.070 ± 0.000 | not detected | 0.070 ± 0.000 | 0.070 ± 0.000 | 0.070 ± 0.000 |
| PDIA6 | 2.986 ± 0.646 | 3.110 ±00.334 | 3.030 ± 0.404 | 2.790 ± 0.191 | 2.576 ± 0.490 | 3.048 ± 0.638 | 2.966 ± 0.538 | 3.200 ± 0.347 |
| PDIA9 | 3.654 ± 0.747 | 3.410 ± 0.589 | 2.940 ± 0.323 | 2.80 ± 0.606 | 2.018 ± 0.487 | 2.088 ± 0.756 | 2.118 ± 0.566 | 2.350 ± 0.784 |
| PDIA10 | 1.850 ± 0.565 | 1.950 ± 0.533 | 1.848 ± 0.125 | 1.173 ± 0.231 | 0.604 ± 0.252 | 0.878 ± 0.229 | 0.776 ± 0.287 | 0.605 ± 0.187 |
| PDIA11 | 0.600 ± 0.110 | 0.868 ± 0.23 | 0.868 ± 0.243 | 0.420 ± 0.104 | 0.580 ± 0.291 | 0.435 ± 0.090 | 0.672 ± 0.254 | 0.535 ± 0.183 |
| PDIA12 | 0.130 ± 0.000 | 0.130 ± 0.000 | 0.130 ± 0.000 | 0.130 ± 0.000 | 0.130 ± 0.000 | 0.130 ± 0.000 | 0.137 ± 0.012 | 0.130 ± 0.000 |
| PDIA13 | not detected | not detected | 0.080 ± 0.000 | not detected | not detected | not detected | 0.080 ± 0.000 | 0.080 ± 0.000 |
| PDIA14 | 0.143 ± 0.065 | 0.175 ± 0.092 | 0.173 ± 0.072 | not detected | 0.110 ± 0.000 | 0.110 ± 0.000 | 0.130 ± 0.000 | 0.110 ± 0.000 |
| PDIA15 | 2.928 ± 0.544 | 2.793 ± 0.313 | 2.738 ± 0.594 | 2.220 ± 0.43 | 2.128 ± 0.396 | 2.330 ± 0.850 | 2.060 ± 0.477 | 2.185 ± 0.259 |
| PDIA16 | 1.094 ± 0.252 | 0.933 ± 0.340 | 1.255 ± 0.230 | 0.440 ± 0.173 | 0.765 ± 0.488 | 0.558 ± 0.274 | 1.250 ± 0.430 | 0.598 ± 0.534 |
| PDIA17 | 10.732 ± 1.024 | 9.200 ± 2.680 | 11.320 ± 2.096 | 8.420 ± 2.399 | 0.423 ± 0.167 | 1.130 ± 0.565 | 0.697 ± 0.537 | 0.375 ± 0.205 |
| PDIA18 | 1.554 ± 0.740 | 1.305 ± 0.576 | 1.305 ± 0.576 | 1.253 ± 0.595 | not detected | not detected | 0.240 ± 0.000 | 0.240 ± 0.000 |
| PDIA19 | 0.130 ± 0.057 | 0.193 ± 0.081 | 0.305 ± 0.900 | 0.133 ± 0.029 | 0.050 ± 0.000 | 0.083 ± 0.029 | 0.078 ± 0.049 | 0.063 ± 0.025 |
Semi-quantitative data for each detected PDI isoform (emPAI) in lysates from breast cancer MCF-7 and MDA-MB-231 cell lines after PDIA1 silencing. Data represent the means ± SD from minimum of 3 consecutive passages (MCF-7, WT—n = 5, shN—n = 4, shPDIA1-1—n = 4, shPDIA1-3—n = 3; MDA-MB-231, WT—n = 5, shN—n = 4, shPDIA1-1—n = 5, shPDIA1-3—n = 4). emPAI was calculated based on the number of observed peptides per proteins normalized by the theoretical number of peptides subtracted from LC-MS/MS data using MascotTM (Matrix Sciences, London, UK).