Figure 6.

G-CSFR knockdown in macrophages promotes (A) increased nitrite levels by fluorometric plate assay, and in adoptive transfer models, (B) an increased NOS2 gene expression, and (C) increased gene expression of BAD, CASP3, and FASL apoptosis genes with simultaneous decreased BCL2 proliferation gene. (D) MC38 incubation with LPS- and IFNγ-treated macrophages also shows increased tumor killing by G-CSFR^−/− macrophages compared to WT by Annexin V staining for flow cytometry. n = 6 for (A), 10 for (B) and (C), and 6 for (D). * p and ** p ≤ 0.05.