FIGURE 7.

NLRP3 inflammasome has limited influence on cell‐free hemoglobin‐induced lung injury after 2 hr. NLRP3KO mice or WT controls were exposed to intra‐tracheal CFH (100 μg). There were no significant differences in (a) total BAL cell counts (p = .284) or (b) BAL protein (p = .263). (c) IL‐1β was significantly higher in WT mice after CFH compared to NLRP3KO mice (p = .007). There were also no NLRP3‐dependent changes in (d–e) histological lung injury (p = .204) or (f–g) myeloperoxidase staining (p = .948) in CFH‐injured lungs. Representative histology images for each treatment group are shown in Panels e (20×) and g (40×). n = 3 for PBS, n = 5 for CFH groups