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. 2020 Oct 9;9(10):965. doi: 10.3390/antiox9100965

Figure 3.

Figure 3

Preventive effect of Mito T and NAC against APAP-induced cellular injury and mitochondrial oxidative stress in a 3D cultured HepG2 cell model. HepG2 cells cultured in NCP (3D-NCP HepG2 cells) were exposed to 15 mM APAP in the presence or absence of Mito-T (10 µM) or NAC (100 µM). The reagents were added just after cell exposure to APAP. (A) Cell viability was measured 24 h after APAP exposure by changes in mitochondrial dehydrogenase activity using the WST-8 assay kit. Each bar represents the mean ± SEM (n = 3). (B) MitoSOX (5 µM) was added for 15 min after 24 h exposure to APAP. The cells were imaged at excitation/emission wavelengths of 490/510 nm and under bright field using a microscope. Random fields from each well were analyzed by microscopy, and the fluorescence of MitoSOX was quantified using ImageJ software (1.47v). Control or vehicle groups were treated with DMEM medium only. Each bar represents the mean ± SEM (n = 5). * p < 0.05, ** p < 0.01 vs. the vehicle group, # p < 0.05 vs. the APAP group.