Title and abstract |
1 |
(a) Indicate the study’s design with a commonly used term in the title or the abstract |
Title and Abstract (p. 1 lines 19–20) |
(b) Provide in the abstract an informative and balanced summary of what was done and what was found |
Abstract (p. 1) |
Introduction |
|
Background/rationale |
2 |
Explain the scientific background and rationale for the investigation being reported |
Introduction (p. 1–2) |
Objectives |
3 |
State specific objectives, including any prespecified hypotheses |
Objectives p. 1, lines 71–73. No prespecified hypotheses included. |
Methods |
|
Study design |
4 |
Present key elements of the study design early in the paper |
Abstract, Introduction, and Methods |
Setting |
5 |
Describe the setting, locations, and relevant dates, including periods of recruitment, exposure, follow-up, and data collection |
Exposure and follow-up not relevant to study design. Recruitment data collection dates included (Methods, p. 3, line 97). |
Participants |
6 |
(a) Give the eligibility criteria and the sources and methods of selection of participants |
Methods, p. 3, lines 81–83) |
Variables |
7 |
Clearly define all outcomes, exposures, predictors, potential confounders, and effect modifiers. Give diagnostic criteria, if applicable |
Outcomes defined throughout Methods section. Unmeasured potential confounders discussed (Discussion, line 256–262 and lines 269–272). Exposures and diagnostic criteria not applicable to current study. |
Data sources/measurement |
8 |
For each variable of interest, give sources of data and details of methods of assessment (measurement). Describe comparability of assessment methods if there is more than one group |
Presented throughout the Methods section |
Bias |
9 |
Describe any efforts to address potential sources of bias |
Methods, particularly lines 85–96 |
Study size |
10 |
Explain how the study size was arrived at |
Only an estimate of the adequacy of total population was considered, with broader convenience sampling based on available study timeline (Methods, lines 97–101) |
Quantitative variables |
11 |
Explain how quantitative variables were handled in the analyses. If applicable, describe which groupings were chosen and why |
Data handling described in Methods (lines 86–128) |
Statistical methods |
12 |
(a) Describe all statistical methods, including those used to control for confounding variables |
Methods (lines 129–134) |
(b) Describe any methods used to examine subgroups and interactions |
Methods and Results |
(c) Explain how missing data were addressed |
Not applicable (see Results line 136) |
(d) If applicable, describe analytical methods taking account of sampling strategy |
Not carried out |
(e) Describe any sensitivity analyses |
Not carried out. Potential unmeasured confounders discussed |
Results |
|
Participants |
13 |
(a) Report numbers of individuals at each stage of study—e.g., numbers potentially eligible, examined for eligibility, confirmed eligible, included in the study, completing follow-up, and analysed |
No data collected on potential eligibility or number of individuals who declined to take part. |
(b) Give reasons for non-participation at each stage |
Not applicable (see 13 (a)). |
(c) Consider use of a flow diagram |
Not applicable (see 13 (a)) |
Descriptive data |
14 |
(a) Give characteristics of the study participants (e.g., demographic, clinical, social) and information on exposures and potential confounders |
Results Table 1, Table 2, Table 3, Table 4 and Table 5
|
(b) Indicate the number of participants with missing data for each variable of interest |
Not applicable (see 13 (a)) |
Outcome data |
15 |
Report numbers of outcome events or summary measures |
Results Table 1, Table 2, Table 3, Table 4 and Table 5
|
Main results |
16 |
(a) Give unadjusted estimates and if applicable, confounder-adjusted estimates and their precision (e.g., 95% confidence interval). Make clear which confounders were adjusted for and why they were included |
Confounder-adjusted estimates not applicable to the current study design |
(b) Report category boundaries when continuous variables were categorized |
BMI categories used noted in Results (Table 3, lines 159–161). |
(c) If relevant, consider translating estimates of relative risk into absolute risk for a meaningful time period |
Not applicable |
Other analyses |
17 |
Report other analyses done—e.g., analyses of subgroups and interactions and sensitivity analyses |
Analyses of sub-groups described throughout Results. Interaction and sensitivity analyses not carried out. |
Discussion |
|
Key results |
18 |
Summarise key results with reference to study objectives |
Discussion (lines 191–257) |
Limitations |
19 |
Discuss limitations of the study, taking into account sources of potential bias or imprecision. Discuss both the direction and magnitude of any potential bias |
Discussion (lines 241–250, lines 258–264, lines 269–274, lines 275–301) |
Interpretation |
20 |
Give a cautious overall interpretation of results considering objectives, limitations, multiplicity of analyses, results from similar studies, and other relevant evidence |
Major interpretations of results presented in lines 317–318 of Conclusions. |
Generalisability |
21 |
Discuss the generalisability (external validity) of the study results |
Considered in relation to broader limitations of the study design (lines 275–301). Conclusions related to this are presented on lines 318–322 |
Other information |
|
Funding |
22 |
Give the source of funding and the role of the funders for the present study and if applicable, for the original study on which the present article is based |
Presented post-Conclusions (lines 329–330) |