Fig. 3.

Erlotinib and cisplatin combination treatment inducing apoptosis and reducing anti-apoptotic gene expression. The EOC cells were treated with cisplatin (0.1 μg) and erlotinib (2 μM) for 48 h, then total RNA was harvested for qRT-PCR analysis. Gene expression levels were normalized to HPRT1. Erlotinib in combination with cisplatin dramatically diminished the gene expression of pro-apoptotic genes in SKOV3 (A) and OVCAR3 (B) cells. Anti-apoptotic genes expression levels were meaningfully depleted in erlotinib/cisplatin treatment in SKOV3 (C) and OVCAR3 (D) cell lines. ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001