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. 2020 Mar 30;24(6):370–378. doi: 10.29252/ibj.24.6.365

Table 1.

Nucleotide sequences of the primers used for qRT-PCR

Gene Accession number Forward primer Reverse primer Amplicon
size (bp)
HPRT1 NM_000194 GGACAGTACGGGAGATCACAG GCACTAATTTCCTTCAGGGATCG 111
FOXO1 NM_002015 TGATAACTGGAGTACATTTCGCC CGGTCATAATGGGTGAGAGTCT 80
FOXO3 NM_001455 ACGGCTGACTGATATGGCAG CGTGATGTTATCCAGCAGGTC 85
FOXO4 NM_005938 CACGTATGGATCCGGGGAAT CCCCTCCGTGTGTACCTTTTC 191
P21 NM_000389 CCTGTCACTGTCTTGTACCCT GCGTTTGGAGTGGTAGAAATCT 130
P27 NM_004064 AACGTGCGAGTGTCTAACGG CCCTCTAGGGGTTTGTGATTCT 209
BAX NM_001291428 CGAGAGGTCTTTTTCCGAGTG GTGGGCGTCCCAAAGTAGG 242
BCL2 NM_000633 CGGTGGGGTCATGTGTGTG CGGTTCAGGTACTCAGTCATCC 90
ABCG2 NM_004827 TGAGCCTACAACTGGCTTAGA CCCTGCTTAGACATCCTTTTCAG 75
ABCB1 NM_000927 TTGCTGCTTACATTCAGGTTTCA AGCCTATCTCCTGTCGCATTA 105
ABCC NM_004996 CTCTATCTCTCCCGACATGACC AGCAGACGATCCACAGCAAAA 94
cIAP1 NM_001166 AGCACGATCTTGTCAGATTGG GGCGGGGAAAGTTGAATATGTA 102
XIAP NM_001167 ATAGTGCCACGCAGTCTACAA AGATGGCCTGTCTAAGGCAAA 101
MCL1 NM_021960 TGCTTCGGAAACTGGACATCA TAGCCACAAAGGCACCAAAAG 135
CCND1 NM_053056 GCTGCGAAGTGGAAACCATC CCTCCTTCTGCACACATTTGAA 135
MYC NM_002467 GTCAAGAGGCGAACACACAAC TTGGACGGACAGGATGTATGC 162
Survivin NM_001168 CCAGATGACGACCCCATAGAG TTGTTGGTTTCCTTTGCAATTTT 152
EGF NM_001963 TGTCCACGCAATGTGTCTGAA CATTATCGGGTGAGGAACAACC 133
HB-EGF NM_001945 ATCGTGGGGCTTCTCATGTTT TTAGTCATGCCCAACTTCACTTT 86
AREG NM_001657 GAGCCGACTATGACTACTCAGA TCACTTTCCGTCTTGTTTTGGG 121
EREG NM_001432 GTGATTCCATCATGTATCCCAGG GCCATTCATGTCAGAGCTACACT 120
BTC NM_001729 CCTGGGTCTAGTGATCCTTCA CTTTCCGCTTTGATTGTGTGG 131
HER3 NM_001982 GGTGATGGGGAACCTTGAGAT CTGTCACTTCTCGAATCCACTG 80
HER2 NM_001005862 CAGTGCAGCACAGAGACTCA CCGGTGCACACTCACTTTTG 103
EGFR NM_001346897 AGACAGCTTCTTGCAGCGAT TTCCAGACAAGCCACTCACC 105
HRG1A NM_013964 AAACCAAGAAAAGGCGGAGGAGCT GAGGGCGATGCAGATGCCGG 70
HRG1B NM_013956 GCCAGCTTCTACAAGCATCTTGGGA GGAGGGCGATGCAGATGCCG 97