Table 1.
Retention times, m/z of precursor ion, internal standard (IS), and linear range of studied FAs.
| Compound | Retention Time (min) | IS * | Precursor Ion (m/z) | Linear Range (μmol/L) | LOD # | LOQ § |
|---|---|---|---|---|---|---|
| Octanoic acid, C8:0 | 1.6 | (A) | 455.3 | 0.75–75 | 9.9 | 33.0 |
| Decenoic acid, C10:1 | 2.0 | (B) | 481.3 | - | ||
| Decanoic acid, C10:0 | 2.5 | (B) | 483.3 | 3.75–375 | 14.0 | 46.7 |
| Lauroleic acid, C12:1 | 2.8 | (C) | 509.2 | - | ||
| Lauric acid, C12:0 | 3.2 | (C) | 511.2 | 7.5–750 | 12.7 | 42.3 |
| Tetradecadienoic acid, C14:2 | 3.0 | (D) | 535.4 | - | ||
| Myristoleic acid, C14:1 | 3.4 | (D) | 537.4 | - | ||
| Myristic acid, C14:0 | 3.8 | (D) | 539.4 | 7.5–750 | 9.0 | 30.0 |
| Hexadecenoic acid, C16:1w9 | 3.9 | (E) | 565.4 | 7.5–750 | 8.6 | 28.7 |
| Palmitic acid, C16:0 | 4.7 | (E) | 567.3 | 60–6000 | 4.2 | 14.0 |
| Stearidonic acid, C18:4 | 3.5 | (F) | 587.4 | - | ||
| α-Linolenic acid, C18:3w3 | 3.8 | (F) | 589.4 | 3.75–375 | 5.1 | 17.0 |
| Linoleic acid, C18:2w6 | 4.2 | (F) | 591.4 | - | ||
| Oleic acid, C18:1w9 | 4.9 | (F) | 593.4 | - | 5.9 | 19.7 |
| Stearic acid, C18:0 | 5.5 | (F) | 595.4 | 30–3000 | 6.8 | 22.7 |
| EPA, C20:5w3 | 3.8 | (H) | 613.4 | 1.9–188 | 14.0 | 46.7 |
| Arachidonic acid, C20:4w6 | 4.2 | (H) | 615.4 | 22.5–2250 | 8.6 | 28.7 |
| h-γ-Linolenic acid, C20:3w6 | 4.5 | (H) | 617.4 | - | ||
| Eicosadienoic acid, C20:2 | 5.1 | (H) | 619.4 | - | ||
| Gondoic acid, C20:1 | 5.6 | (H) | 621.4 | - | ||
| Arachidic acid, C20:0 | 6.1 | (H) | 623.4 | 0.75–75 | 9.0 | 30.0 |
| Pristanic acid, C19:0 branched | 5.3 | (G) | 609.2 | 0.75–75 | 11.4 | 38.0 |
| Phytanic acid, C20:0 branched | 5.7 | (H) | 623.2 | 0.75–75 | 9.6 | 32.0 |
| DHA, C22:6w3 | 4.1 | (I) | 639.2 | - | ||
| DPA, C22:5w3 | 4.6 | (I) | 641.2 | 0.75–75 | 14.3 | 47.7 |
| DTA, C22:4w6 | 4.9 | (I) | 643.2 | - | ||
| Docosatrienoic acid, C22:3 | 5.5 | (I) | 645.2 | - | ||
| Docosadienoic acid, C22:2 | 5.8 | (I) | 647.2 | - | ||
| Docosenoic acid, C22:1 | 6.2 | (I) | 649.2 | - | ||
| Docosanoic acid, C22:0 | 6.6 | (I) | 651.2 | 3.75–375 | 10.3 | 34.3 |
| Nervonic acid, C24:1 | 6.6 | (J) | 677.3 | - | ||
| Tetracosanoic acid, C24:0 | 7.1 | (J) | 679.3 | 3.75–375 | 9.8 | 32.7 |
| Hexacosenoic acid, C26:1 | 7.1 | (K) | 705.3 | - | ||
| Hexacosanoic acid, C26:0 | 7.9 | (K) | 707.3 | 0.15–15 | 15.1 | 50.3 |
| Octacosenoic acid, C28:1 | 7.9 | (K) | 733.3 | - | ||
| Montanic acid, C28:0 | 9.1 | (K) | 735.3 | - |
* (A) 13C4 C8:0 at 7.5 μmol/L, (B) d3-C10 at 37.5 μmol/L, (C) d3-C12 at 75 μmol/L, (D) d3-C14 at 75 μmol/L, (E) d4-C16 at 600 μmol/L, (F) d3-C18 at 300 μmol/L, (G) d3-PRA at 7.5 μmol/L, (H) d3-PHA at 7.5 μmol/L, (I) d4-C22 at 37.5 μmol/L, (J) d4-C24 at 37.5 μmol/L, (K) d4-C26 at 1.5 μmol/L; # LOD, limit of detection (pmol/injection); § LOQ, limit of quantitation (pmol/injection).