Figure 5.

DBSCs proliferation and mineral matrix deposition on large pore scaffolds. (a–d) Representative phase contrast pictures of DBSCs treated with Pol or Ctr for for 14 days (a,b) and 21 days (c,d) in osteogenic conditions on scaffolds presenting pores of large dimensions (1.15 mm). Scale bar = 100 μm. (e,f) ARS (red staining) displayed mineral matrix deposition by DBSCs after 28 days of culture. (g) The graph shows ARS quantification using the OD as mean percentage ± SD and is representative for three independent experiments performed in quadruplicates. Student’s t-test was used for single comparisons. The biomaterial pores of a representative experiment were chosen for the figure.