Figure 1.
Comparison of the antibody titer of anti-scorpion and spider anti-venoms and anti-toxins against spider (rLiD1) and scorpion toxin (rHNC) and Hemiscorpius lepturus (HL) venom. Reactivity against anti-HL (A), anti-Loxosceles (B), anti-rLiD1 (C) antibodies. The antibody titer was considered as the highest dilution of serum capable of resulting in a reading greater than 1.0. For this calculation, eight different dilutions of each one of the antibodies (anti-HL, anti-Loxosceles and anti-rLiD1) were used (1:100, 1:500, 1:1000, 1:5000, 1:10,000, 1:50,000, 1:100,000, 1:250,000). For competition ELISA, the different antigens (10−6 M) were incubated for 30 min at 37 °C with each dilution of each antibody.