Figure 2.

Neutralization of HL venom- rHNC- and rLiD1-induced hemolytic effect by immune sera. (A) Whole H. lepturus venom (HL), rHNC and rLiD1 were tested from 400 to 10 ng/mL for their capacity to lyse human RBCs in the presence of control-matched serum as a source of complement. Results are expressed as percentages of hemolysis. (B) Neutralization of RBCs hemolysis by anti-HL, anti-Loxosceles, and anti-rLiD1 immune sera. Samples were mixed with diluted anti-sera (1:50) and incubated with 2% RBCs. Percentages of neutralization were determined as described in methods. Results are representative of three different experiments expressed as the mean of duplicates ± tandard deviation.