Figure 3.

Assessment of rHNC and rLiD1 enzymatic activity. Various concentrations of toxins were incubated with 100 µM of BSA-LPC, SM, and PC for 1 h at 37 °C. The formed choline, oxidized to betaine, was determined fluorometrically. Results are representative of three independent experiments and are expressed as the mean of triplicates ± standard deviation.