Skip to main content
. 2020 Sep 30;12(10):2824. doi: 10.3390/cancers12102824

Figure 1.

Figure 1

Overview of Yeast Surface Display (YSD) platform design. Native antibody scFv is cloned into YSD vector and yeast cells induced to express native scFv (1). The scFv fragment is PCR amplified (2) followed by error prone PCR to generate random mutagenesis (3). Mutated scFvs fragments are cloned into YSD vectors (4), transformed into yeast cells, and then induced to express scFv on cells surface (5). The scFv expression and antigen binding are measured by Fluorescence-Activated Cell Sorting (FACS) and top Ag+Ab+-double positive clones can be sorted out (6).