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. 2020 Sep 30;12(10):2824. doi: 10.3390/cancers12102824

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Overview of full-antibody expression and subsequent characterization. (a) Full length human IgG antibodies were generated from native and selected-mutants scFv clones. Variable heavy chain (VH) and variable light chain (VL) sequences were cloned into hIgG1/kappa vectors (1). The 293A cells were transfected to produce the full length antibodies (2). Antibodies were purified from cell media (3). (b) Full length antibodies were characterized for their affinity by bio-layer interferometry (BLI) (4), specificity by ELISA, and glycan microarray (5), cancer cell binding (6), and complement-dependent cellular cytotoxicity (7).