Skip to main content
. 2020 Oct 12;12(10):2933. doi: 10.3390/cancers12102933

Figure 1.

Figure 1

Combined dendrogenin A (DDA) and daunorubicin synergistically induce the death of AML cells. (A) Combination Index: CI < 0.80 indicates synergy of DDA with daunorubicin in KG1a, KG1, MV4-11 cells (B) Intra-cellular daunorubicin concentration was quantified by flow cytometry after 24 h of DDA treatment at 2.5 µM. DATA were represented as % of intra-cellular daunorubicin concentration relative to vehicle treated cells. Bars are mean ± SD of 3 independent experiments. (C) PgP membrane expression was measured by flow cytometry after 48 h of DDA treatment at 2.5 µM in four primary AML sample. Data were represented as % of PgP membrane expression relative to vehicle treated cells. (D) Intra-cellular daunorubicin concentration was quantified after 24 h of DDA treatment at 2.5 µM in KG1-shctrl and Kg1-ShLXRβ cells. Data were represented as % of intra-cellular daunorubicin concentration relative to vehicle treated cells. Points are mean ± SD of 3 independent experiments. (E) PgP membrane expression was measured after 24 h of DDA treatment at 2.5 µM in KG1Shctrl and KG1-ShLXRβ. Data were represented as % of PgP membrane expression relative to vehicle treated cells. Bars are mean ± SD of 3 independent experiments. (F) Intra-cellular IDA concentration was quantified by flow cytometry after 24 h of DDA treatment at 2.5 µM. Data were represented as % of intra-cellular idarubicin concentration relative to vehicle treated cells. Bars are mean ± SD of 3 independent experiments. * p < 0.05.