. 2020 Oct 12;9(10):833. doi: 10.3390/pathogens9100833

Table 3.

PRV-1 RNA levels (Ct values) in 26 cell lines exposed to Canadian (PRV-1a) and Norwegian (PRV-1b) red blood cells homogenate in single and multiple passages virus isolation assays.

Species	Cell Line	Virus Isolation Assays	Passage Number of Collected Samples (Initial–Final)	Cumulative Days Post-Inoculation (Initial–Final)	Ct Value (Initial–Final)
Species	Cell Line	Virus Isolation Assays	Passage Number of Collected Samples (Initial–Final)	Cumulative Days Post-Inoculation (Initial–Final)	Canada PRV	Norway PRV
American Eel	EelB	Single cell culture passage	1	2–14	30.7–30.9	32.6–32.7
	EelB	Sub-cultivating only exposed cells	1–2	22–43	27.6–28.9	27.2–30.1
	PBLE	Single cell culture passage	1	2–14	30.5–29.4 *	33.7–29.2 *
	PBLE	Sub-cultivating only exposed cells	1–2	22–43	27.4–27.2	26.9–29.4
Arctic Charr	ACBA	Supernatant transfer	1–2	14–28	25.2–34.2	27.6 *–N
Arctic Charr	ACBA	Sub-cultivating only exposed cells	1–2	22–43	28.2–29.1	26.4–30.4
Atlantic Salmon	ASCF	Single cell culture passage	1	2–14	29.9–29.4	31.9–32.4
	ASCF	Sub-cultivating only exposed cells	1–2	15–44	25.7–24.8	27.8–31.5
	ASHe	Single cell culture passage	1	2–14	30.8–30.9	33.5–32.1
		Sub-cultivating with fresh cells	1–2	14–28	31.4–31.3	No data
		Supernatant transfer	1–2	26–40	31.4–N	35.5 –37.4
		Cell lysate transfer	1–2	14–28	22.2–25.4 **	25.7–27.1
		Sub-cultivating only exposed cells	1–2	15–44	28.8–31.0	28.8–32.8
	ASimf20	Single cell culture passage	1	2–14	28.4–28.4	32.1–32.2
		Supernatant transfer	1–2	26–40	25.0–37.1 *	27.2–N
		Cell lysate transfer	1–2	14–28	20.9–25.3	25.5–27.1
		Sub-cultivating only exposed cells	1–2	15–43	25.8–27.5	25.2–31.2
	ASP309	Supernatant transfer	1–2	26–40	24.4–31.2	28.2–34.9
	BAASf	Single cell culture passage	1	2–14	28.7–28.7	31.6–31.6
		Sub-cultivating with fresh cells	1–2	14–28	27.0–30.4	No data
		Cell lysate transfer	1–2	14–28	22.1–26.7	25.1–27.6 *
		Sub-cultivating only exposed cells	1–2	15–44	27.6–29.8	27.2–31.4
Chinook Salmon	CHSE-214	Supernatant transfer	1–2	14–28	27.3–33.2	30.5–N
	CHSE-214	Cell lysate transfer	1–2	14–28	24.0–27.0 *	24.9–N
Fathead Minnow	EPC	Supernatant transfer	1–2	14–28	24.9–33.9 *	29.2–33.9
	EPC	Cell lysate transfer	1–2	14–28	25.5–28.2	27.1–31.9
Gar	GarL	Single cell culture passage	1	2–14	29.7 *–27.6	33.2 *–30.2
Pacific herring	PHL	Sub-cultivating with fresh cells	1–2	14–28	26.2–30.2	No data
Rainbow Trout	RTgill-W1	Supernatant transfer	1–2	14–28	26.9–32.4	29.8–33.8
		Cell lysate transfer	1–2	14–28	25.5–26.5	27.5–30.0
		Sub-cultivating only exposed cells	1–2	14–44	26.7–28.1	N–N
	RTgutGC	Single cell culture passage	1	2–14	30.8–28.3	31.0–32.0
		Sub-cultivating with fresh cells	1–2	14–28	26.0–N	No data
		Cell lysate transfer	1–2	14–28	24.6–28.1	26.9 *–30.2
		Sub-cultivating only exposed cells	1–2	14–44	27.4–29.2	28.5–33.2
	RTHDF	Single cell culture passage	1	2–14	31.7–30.2	34.0–34.9 *
		Sub-cultivating with fresh cells	1–2	14–28	31.6–31.3	No data
		Supernatant transfer	1–2	14–28	26.3 *–33.8	29.2–33.1
		Cell lysate transfer	1–2	14–28	22.9–26.6	26.5–28.7
		Sub-cultivating only exposed cells	1–2	14–44	28.1–29.4	27.0–31.6
	RTH-149	Single cell culture passage	1	2–14	29.5–26.6	33.0 *–N
	RTH-149	Sub-cultivating only exposed cells	1–2	15–43	24.7–24.7	28.9–32.4
	RTL-W1	Supernatant transfer	1–2	14–28	28.2 *–33.6	27.0–N
		Cell lysate transfer	1–2	14–28	24.9–27.0	26.9–N
		Sub-cultivating only exposed cells	1–2	14–44	28.0–28.5	28.7–33.5
	RTS11	Cell lysate transfer	1–2	14–28	22.6–27.0	26.9–28.8
	RTS11	Sub-cultivating only exposed cells	1–2	15–43	22.6–23.8	25.8–26.0
Stickleback (Threespined)	STB5 Gill	Single cell culture passage	1	2–14	31.7–30.5	31.6–33.8
	STB5 Gill	Sub-cultivating with fresh cells	1–2	14–28	27.1–32.7	No data
	STB6 Gill	Sub-cultivating with fresh cells	1–2	14–28	23.8–28.3	No data
	STBH	Sub-cultivating with fresh cells	1–2	14–38	25.0–27.2	No data
Sturgeon	SB3	Supernatant transfer	1–2	14–28	24.4–32.7 *	28.7–N
Walleye	WE-cfin11f	Sub-cultivating only exposed cells	1–2	16–43	23.7–25.6	24.9–29.4
Yellow Perch	YPF5	Sub-cultivating only exposed cells	1–2	22–43	24.3–28.6	28.7–31.2
Zebrafish	ZEB2J	Single cell culture passage	1	2–14	27.7 *–27.6	31.0–32.2
	ZEB2J	Sub-cultivating only exposed cells	1–2	16–43	26.1–28.4	27.1–30.8
	ZSSJ	Sub-cultivating with fresh cells	1–2	14–28	26.2–30.1	No data

Ct—Average of two biological replicates. N—Samples with Ct value equal to or greater than 35.0 and those without Ct values are considered as negative. * One of two biological replicates was negative. ** Only one biological replicate. No data—experiment not performed.