Table 5.

PRV-1 RNA levels (Ct values) in eight cell lines co-administered with either Canadian (PRV-1a) or Norwegian (PRV-1b) red blood cell homogenates together with either CSV ¹ or IPNV ².

Cell Cultures			Ct 3 in Cultures at 2 Times after Initiation of Exposure
Cell Line	RBC Homogenates	Co-Infected Virus	Passage 1 (13 to 18 Days 4)	Passage 2 [26 to 32 Cumulative Days 5 (12 to 14 Days after Passaging)]
EPC	Canada	CSV	22.4 *	32.0 *
	Norway	CSV	24.5	33.7
	Canada	IPNV	26.8	32.4
	Norway	IPNV	29.9	Negative
ASHe	Canada	IPNV	25.3	27.8
	Norway	IPNV	25.2	27.5
BAASf	Canada	IPNV	26.7	29.0
	Norway	IPNV	26.6	30.0
CHSE-214	Canada	CSV	21.5	30.4
	Norway	CSV	23.4	30.2
	Canada	IPNV	25.1	31.8
	Norway	IPNV	26.1	30.2
RTHDF	Canada	CSV	26.8	32.2
	Norway	CSV	29.3	Negative
	Canada	IPNV	27.9	32.6
	Norway	IPNV	28.0	32.8
RTgill-W1	Canada	CSV	24.7	28.8
	Norway	CSV	28.0	33.1
	Canada	IPNV	25.0	30.7
	Norway	IPNV	26.9	28.6
RTL-W1	Canada	CSV	24.1	31.6
	Norway	CSV	28.5	Negative
RTS11	Canada	CSV	Negative	32.8 **
	Norway	CSV	25.8 **	33.6 **
	Canada	IPNV	23.9	30.4 **
	Norway	IPNV	24.9	30.4 **

¹ CSV =chum salmon reovirus at 10^4.3 TCID₅₀/mL. ² IPNV= infectious pancreatic necrosis virus at 10^8.1 TCID₅₀/mL. ³ Ct =cycle threshold; samples with Ct value ≥ than 35 or without Ct values are scored negative. ⁴ For some replicate flasks after 13–18 days, RNA was extracted for RT-qPCR. For other replicate flasks after 13–18 days, the medium was collected & applied to fresh cultures and incubated further. ⁵ After 12–14 days of incubation with medium from passage 1, RNA was extracted for RT-qPCR. * All values are means of two biological replicates. ** One of two biological replicates was negative.