Figure 7.

Virus family presence contained (A) in NTC and (B) in TM (other than the target virus) in each triplicate from the different extraction methods. The heatmap showed the viral family read count associated with the kitome (the presence of reads associated with other viruses than the targeted viruses) normalized in log₁₀(RPM) in each NTC and TM between the different extraction methods. A gradient of colors was defined from gray (no count) to blue (few counts) to red (highest counts). During the automated extractions, the NTCs were interspersed between samples (i.e., 3 NTCs per batch). For manual extraction, only one NTC was added. In addition to the NTC, transport medium was added. Analyses were performed on the family taxonomical level. A sample was considered to be positive for a particular virus when the log₁₀(RPM) of this virus exceeded 1. HSV: herpes simplex virus; RSV: respiratory syncytial virus; NTC: no template control; TM: transport medium; RPM: reads per million.