Table 2.
Primers for real-time PCR assay (used in this study).
| Target Species | Primer | Sequence (5′-3′) | Reference |
|---|---|---|---|
| Total bacteria | F R |
CGGCAACGAGCGCAACC CCATTGTAGCACGTGTGTAGCC |
[19] |
| Fungi | F R |
GAGGAAGTAAAAGTCGTAACAAGGTTTC CAAATTCACAAAGGGTAGGATGATT |
[19] |
| Protozoa | F R |
GCTTTCGWTGGTAGTGTATT CTTGCCCTCYAATCGTWCT |
[20] |
| Methanogens | F R |
TTCGGTGGATCDCARAGRGC GBARGTCGWAWCCGTAGAATCC |
[20] |
| B. fibrisolvens | F R |
ACACACCGCCCGTCACA TCCTTACGGTTGGGTCACAGA |
|
| C.proteoclasticum | F R |
TCCGGTGGTATGAGATGGGC GTCGCTGCATCAGAGTTTCCT |
|
| F. succinogenes | F R |
GTTCGGAATTACTGGGCGTAAA CGCCTGCCCCTGAACTATC |
[21] |
| R. albus | F R |
CGGCAACGAGCGCAACCC CCATTGTAGCACGTGTGTAGCC |
[22] |
| R. flavefaciens | F R |
CGAACGGAGATAATTTGAGTTTACTTAGG CGGTCTCTGTATGTTATGAGGTATTACC |
[23] |