Figure 9.
Diet supplementation of HFD fed rats with EVOO, ROO, PE-OL-B, PE-P-B, and PE-GM-B affects vessel sprouting formation from aortic rings. Aorta rings were treated with VEGF (25 ng/mL). Vessel sprouting formation was determined as the number of grid units covering the surface occupied by pseudo-capillaries in each well and expressed as vessel length. n = 4 rats. * p ≤ 0.05 vs. STD; # p ≤ 0.05 vs. HFD. Each experimental point is run at least in triplicate. Scale bar 100 µm.