Figure 3.

V. nonalfalfae growth inhibition on PDA supplemented with different concentrations of p-coumaric acid. Two isolates of V. nonalfalfae were selected for comparison: (a) Rec, mild pathotype M in the left panel and (b) T2, lethal pathotype PV1 in the right panel. Images were taken 14 days after mycelial disc inoculation on PDA plates supplemented with p-coumaric acid, dissolved in 20% methanol at six different concentrations (1, 10, 100, 1000, 1500, and 2000 ppm; depicted from top in a clockwise direction) or with solvent control (middle plate). All experiments were performed in six replicates.