Table 4.
Estimated IC50 values of polyphenolic extracts with 95% confidence intervals (CI) from hop roots and stems against V. nonalfalfae in vitro growth.
| Extract | Strain | ϕ1, asymptote | IC50 (mg/L) |
|---|---|---|---|
| CE stems | Rec | 87 (CI 79−95) | 148 (CI 121−246) |
| T2 | 96 (CI 88−103) | 183 (CI 137−530) | |
| WT stems | Rec | 84 (CI 76−91) | 212 (CI 141−604) |
| T2 | 106 (CI 98−113) | 130 (CI 113−163) | |
| CE roots | Rec | 84 (CI 77−90) | 1683 (CI 1485−1911) |
| T2 | 86 (CI 80−92) | 1172 (CI 1041−1308) | |
| WT roots | Rec | 89 (CI 83−95) | 1804 (CI 1617−2032) |
| T2 | 82 (CI 76−88) | 1223 (CI 1088−1365) |
Dose response sigmoid curves were defined by three parameters: ϕ1, upper asymptote; ϕ2, log 10 of concentration at which a bend is achieved at ϕ1/2; and ϕ3, the rate of decrease of the relative diameter after the bend. Analysis showed that the same value (−0.12, CI −0.15 to −0.09) can be attributed to ϕ3, while parameters ϕ1 and ϕ2 varied between treatments. For ϕ2, antilog values are listed and represent estimated IC50 with corresponding 95% confidence intervals (CI). Rec, mild V. nonalfalfae strain Rečica, T2, lethal V. nonalfalfae strain [11].