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. 2020 Oct 6;11(10):1169. doi: 10.3390/genes11101169

Figure 4.

Figure 4

Lentiviral-mediated basal cell detargeting of EGFP following differentiation at an air–liquid interface. Primary human basal cells were transduced with LV-dt/EGFP-miRT or LV-dt/EGFP-RmiRT vectors and expanded for 1–2 days before seeding for differentiation in air–liquid interface (ALI) cultures. (A,B) Confocal microscopic images of (A) LV-dt/EGFP-miRT and (B) LV-dt/EGFP-RmiRT transduced ALI culture using sections immunostained for KRT5 (keratin-5) and imaged for KRT5, EGFP, and Tomato expression with DAPI to mark nuclei. Dual and single channel images are shown. Enlarged boxed regions in (A,B) are shown in the middle column. Scale bar, 50 μm.