Table 1.

Distribution of cell types in differentiated ALI cultures.

Vector	% Basal Cells (TRP63+)	% Ionocytes (BSND+)	% Ciliated Cells (Ac-Tubulin+)	% Goblet Cells (MUC5AC+)
LV-dt/EGFP-RmiRT	21.8+/−3.8 *	0.82+/−0.02	44.3+/−1.6	11.2+/−0.8
LV-dt/EGFP-miRT	22.4+/−1.5	0.72+/−0.04	41.5+/−0.9	21.9+/−1.2
p-value **	0.7768	0.0388	0.1567	<0.0001

The percentage of viable cells positive for each of the phenotypic cellular markers is shown (TRP63-basal cells; BSND-ionocytes; acetylated tubulin-ciliated cells; MUC5AC-goblet cells). Cells not positive for any of the four antibodies were 21.9% and 12.5% for LV-dt/EGFP-RmiRT- and LV-dt/EGFP-miRT-transduced epithelia, respectively. * Mean +/-SEM. ** Statistical comparisons by Welch’s t test.