Levels of cytokines IL-10 (A,C) and TNFα (B,D) from stimulated MLN cells and PBMCs. MLN cells and PBMCs were stimulated with 10 µg/mL LPS (A,B) or 5 µg/mL Con-A (C,D), or left unstimulated (cell culture medium only) for 24 h. Significant differences between the treatment groups are indicated by asterisks (**; p < 0.01 and *; p < 0.05). Letters (a, b) represent significant differences (p < 0.05) between time points. Every dot represents a single animal from a different pen (n = 7 or 8 per treatment group) and error bars represent standard deviations. Statistical analysis was performed for every time point (T-test) and over time (Two-way ANOVA). Data were checked for normal distribution and equal variances and log-transformed when required.