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. 2020 Oct 13;8(10):1573. doi: 10.3390/microorganisms8101573

Table 1.

Antibodies used to identify DC subsets, NK cells and T cell subsets in PBMCs and mesenteric lymph node (MLN) cells.

Antibody Host/Isotype Clone Fluorochrome Company Dilution
CD14 Mouse, IgG2b MIL-2 FITC Bio-Rad 1:50
CD172a Mouse, IgG2b 74-22-15A PE BD Biosciences 1:40
CD4a Mouse, IgG2b 74-12-4 PerCP-Cy5.5 BD PharmingenTM 1:320
CADM1 Chicken, IgY 3E1 Biotin MBL 1:200
Streptav. n/a n/a BV421 BD HorizonTM 1:50
CD152 * Mouse, IgG2a n/a APC Ancell 1:320
CD3ε Mouse, IgG2a BB23-8E6-8C8 PE-CyTM7 BD PharmingenTM 1:160
CD4a Mouse, IgG2b 74-12-4 PerCP-Cy5.5 BD PharmingenTM 1:320
CD8a Mouse, IgG2a 76-2-11 FITC BD PharmingenTM 1:10
FoxP3 Rat, IgG2a FJK-16s Alexa Fluor® 700 ** eBioscienceTM 1:20
Ki67 Mouse, IgG1 B56 BV421 ** BD HorizonTM 1:80
CD25 Mouse, IgG1 K231.3B2 Purified *** Bio-Rad 1:200
γδ T cells Rat, IgG2a MAC320 PE BD PharmingenTM 1:20

*; Human CD152 (CTLA-4) murine Ig fusion protein was used to detect CD80 and CD86 on DC subsets. **; Antibodies against intracellular antigens. ***; The antibody CD25 (purified) was labelled with the ReadiLink™ Rapid iFluor™ 647 Antibody Labeling Kit (Aat Bioquest, Sunnyvale, CA, USA).