. 2020 Oct 13;8(10):1573. doi: 10.3390/microorganisms8101573

Table 3.

Cell analysis of DCs, NK cells and T cells in MLN cells between treatment groups over time.

		Unpaired T-Test			Two-Way ANOVA
Subset	Phenotype	D27	D44	D70
Subset	Phenotype	p-value [CI]	p-value [CI]	p-value [CI]
pDCs (%)	CD14⁻CD4⁺CD172 a⁺CADM1⁻	ns	ns	ns	T (<0.001)
pDCs (mat.)	CD14⁻CD4⁺CD172 a⁺CADM1⁻CTLA4-Ig⁺	0.034 [3.21 to 67.89] ^↓	ns	0.024 [7.75 to 92.70] ^↓	G (0.007), T (0.001), I (0.08)
cDC1 (%)	CD14⁻CD4⁻CD172 a^lowCADM1⁺	ns	0.011 [−0.11 to −0.02] ^↑	ns	T (<0.001), I (0.028)
cDC1 (mat.)	CD14⁻CD4⁻CD172 a^lowCADM1⁺CTLA4-Ig⁺	ns	ns	ns	T (<0.001)
cDC2 (%)	CD14⁻CD4⁻CD172 a⁺CADM1⁺	ns	ns	ns	T (0.001)
cDC2 (mat.)	CD14⁻CD4⁻CD172 a⁺CADM1⁺CTLA4-Ig⁺	0.084 [−62.71 to 4.50] ^↑	ns	ns	ns
NK cells (%)	CD3⁻CD8 a⁺	n/a	n/a	n/a	n/a
NK cells (act.)	CD3⁻CD8α⁺CD25⁺	n/a	n/a	n/a	n/a
NK cells (Ki67%)	CD3⁻CD8α⁺Ki67⁺	n/a	n/a	n/a	n/a
γδ T cells (%)	CD3⁺TCR-γδ⁺	ns	ns	ns	T (<0.001)
γδ T cells (Ki67%)	CD3⁺TCR-γδ⁺Ki67⁺	0.089 [−15.45 to 1.26] ^↑	ns	ns	T (<0.001), I (0.086)
CTLs (%)	CD3⁺TCR-γδ⁻CD8α⁺	ns	0.035 [−13.03 to −0.60] ^↑	ns	T (0.076)
CTLs (Ki67%)	CD3⁺TCR-γδ⁻CD8α⁺Ki67⁺	ns	ns	ns	T (<0.001)
T helper (%)	CD3⁺TCR-γδ⁻CD4⁺	ns	ns	ns	T (<0.001)
T helper (Ki67%)	CD3⁺TCR-γδ⁻CD4⁺Ki67⁺	0.079 [−12.53 to 0.77] ^↑	ns	ns	T (<0.001), I (0.019)
Mem./Act. (%)	CD3⁺CD4⁺CD8α⁺	ns	ns	ns	T (<0.001)
	CD3⁺CD4 ^highCD8α^low	ns	ns	ns	T (0.002)
	CD3⁺CD4 ^high CD8α^lowki67⁺	ns	ns	ns	T (<0.001)
	CD3⁺CD4 ^lowCD8α^high	ns	ns	ns	ns
	CD3⁺CD4 ^low CD8α^highki67⁺	ns	ns	ns	T (<0.001)
Tregs (%)	CD3⁺TCR-γδ⁻CD4⁺CD25 ^highFoxp3⁺	ns	ns	ns	T (<0.001)
Tregs (Ki67%)	CD3⁺ TCR-γδ⁻CD4⁺CD25 ^highFoxp3⁺Ki67⁺	n/a	n/a	n/a	n/a

Ns; not significant, 0.05 < p < 0.1; trend, p < 0.05; significant. An unpaired Student’s T-test and a Two-way ANOVA were conducted to analyse the group effect per time point, and the effects over time, respectively. G; group effect, T; time effect, I; group-time interaction, act.; activation, mat.; maturation, Mem./Act.; Memory/Activated T cells. Arrows indicate if the values (e.g., percentage of cells) in the treatment group (β-glucan, n = 7 or 8) are significantly higher (^↑) or lower (^↓) than the control group (n = 7 or 8). The 95% confidence intervals (CI) on the difference between the means are shown for all significant results or results which show a trend.