Table 2.
Contents of total soluble phenolic compounds (TSPC; mg ferulic acid equivalents/g of bran) and FA derivatives (µg/g of bran) in WB treated by 13 commercial food-grade glycosidases.
| Enzyme | TSPC | FA | DFA i1 | DFA i2 | DFA dc | VFA |
|---|---|---|---|---|---|---|
| Control | 2.80 ± 0.33 e | 11.64 ± 4.64 f | nd | nd | nd | 3.58 ± 1.03 c |
| Depol 333MDP | 3.30 ± 0.09 de | 53.40 ± 4.48 ef | 24.45 ± 2.16 b | 32.30 ± 0.15 d | 3.30 ± 0.82 d | 4.98 ± 5.81 c |
| Depol 40L | 4.36 ± 0.48 b | 120.10 ± 17.82 de | 19.18 ± 0.85 b | 39.75 ± 0.82 d | 8.63 ± 2.81 d | 20.64 ± 11.46 c |
| Depol 667P | 4.07 ± 0.13 bc | 43.51 ± 3.33 ef | 29.64 ± 2.75 b | 31.02 ± 0.03 d | 2.77 ± 0.75 d | 10.86 ± 1.72 c |
| Depol 670L | 4.38 ± 0.37 b | 528.93 ± 84.87 b | 60.27 ± 3.56 b | 340.92 ± 28.40 c | 73.96 ± 19.76 bc | 63.66 ± 6.46 b |
| Depol 686L | 3.61 ± 0.09 cd | 497.17 ± 67.03 b | 60.77 ± 3.51 b | 419.88 ± 0.27 bc | 85.35 ± 13.46 bc | 76.87 ± 3.73 b |
| Depol 740L | 4.14 ± 0.45 bc | 498.19 ± 82.85 b | 80.51 ± 5.15 b | 498.87 ± 1.65 b | 101.56 ± 12.66 b | 75.53 ± 29.96 b |
| Depol 761P | 4.52 ± 0.09 b | 49.97 ± 1.59 ef | 30.44 ± 0.04 b | 31.56 ± 4.43 d | 4.24 ± 1.66 d | 18.27 ± 13.98 c |
| Depol 793L | 4.27 ± 0.45 b | 240.56 ± 47.63 c | 25.41 ± 3.21 b | 42.57 ± 2.10 d | 15.81 ± 6.10 d | 11.35 ± 1.62 c |
| Pectinase 62L | 3.11 ± 0.10 de | 6.55 ± 1.79 f | 1.86 ± 0.39 b | 5.96 ± 0.06 d | 0.22 ± 0.31 d | 1.77 ± 0.49 c |
| Shearzyme | 4.33 ± 0.41 b | 111.43 ± 11.24 de | 28.00 ± 1.72 b | 38.42 ± 2.25 d | 15.72 ± 2.38 d | 8.20 ± 0.28 c |
| Ultraflo XL | 5.49 ± 0.64 a | 799.50 ± 34.19 a | 555.53 ± 16.12 a | 586.46 ± 13.06 a | 124.37 ± 87.85 a | 233.91 ± 13.96 a |
| Viscoferm | 4.65 ± 0.51 b | 179.75 ± 20.38 cd | 29.90 ± 2.73 b | 37.38 ± 2.58 d | 31.76 ± 10.27 cd | 13.34 ± 1.28 c |
| Viscozyme | 3.16 ± 0.14 de | 51.00 ± 5.35 ef | 12.89 ± 0.83 b | 31.57 ± 3.78 d | 3.99 ± 1.19 d | 8.19 ± 2.52 c |
Data are the mean ± standard deviation of two replicates. Different lowercase letters within a column are significantly different (p < 0.05, Duncan’s test). Abbreviations: decarboxylated form of dihydroferulic acid (DFA dc); dihydroferulic acid isomer 1 (DFA i1); dihydroferulic acid isomer 2 (DFA i2); ferulic acid (FA); total soluble phenolic compounds (TSPC); vanillin ferulic acid (VFA). Hydrolysis conditions were enzyme to WB ratio of 1% (w/w), 40 °C, pH 6, and 20 h.