Table 1.
LC-MS/MS based method development and validation for four standards.
| Marker Compounds | tR a (min) | Mass/Charge (m/z) | Linearity and Range | Sensitivity c | |||
|---|---|---|---|---|---|---|---|
| Regression Equation b | Correlation Coefficient (r2) | Linear Range (μg/g) | LOD (ng/g) | LOQ (ng/g) | |||
| Peimisine | 5.78 | 428.316 | y = 0.0396x + 0.0630 | 0.9923 | 0.1–40 | 0.01 | 0.04 |
| Sipeimine | 8.22 | 430.332 | y = 0.5426x + 0.1531 | 0.9968 | 0.1–40 | 0.02 | 0.06 |
| Peiminine | 10.72 | 430.332 | y = 0.0047x + 0.0010 | 0.9967 | 0.1–40 | 0.97 | 3.23 |
| Peimine | 11.88 | 432.347 | y = 0.0067x + 0.0016 | 0.9977 | 0.1–40 | 0.56 | 1.88 |
a tR: Retention time. b The regression equations are presented as y = mx+c, and y and x are defined as peak area and concentration of the compound, respectively. c LOD: Limit of detection, S/N = 3; LOQ: limit of quantification, S/N = 10.