Table 3.
LC-MS/MS analysis of four isosteroidal alkaloids in in vitro cultures exposed to eight different LED lights, in vitro derived bulblets (3 months old), and commercial Fritillaria cirrhosa D. Don bulbs (wild type, three years old).
| LED Light Treatment |
Plant Material | Isosteroidal Alkaloids (µg/g/dw) |
Total of Four Alkaloids (µg/g/dw) |
|||
|---|---|---|---|---|---|---|
| Peimisine | Sipeimine | Peimine | Peiminine | |||
| CW-5000K | In vitro cultures | ND * | ND | ND | 0.12 ± 0.20 b ** | 0.12 ± 0.20 c ** |
| WW-2700K | In vitro cultures | ND | ND | ND | 0.28 ± 0.27 b | 0.28 ± 0.27 c |
| 7R1G1B | In vitro cultures | ND | ND | ND | 0.19 ± 0.32 b | 0.19 ± 0.32 c |
| 8R1B | In vitro cultures | ND | ND | ND | ND | 0.00 ± 0.00 c |
| 9B | In vitro cultures | ND | ND | ND | 0.60 ± 0.43 b | 0.65 ± 0.45 c |
| 9R | In vitro cultures | 3.65 ± 1.68 b ** | ND | 0.38 ± 0.11 a | 2.40 ± 0.30 a | 6.42 ± 2.06 b |
| 9IR | In vitro cultures | 3.22 ± 3.28 b | ND | 0.05 ± 0.09 b | 2.21 ± 0.87 a | 5.48 ± 3.21 b |
| 3R3B3IR | In vitro cultures | ND | ND | ND | 0.26 ± 0.24 b | 0.26 ± 0.24 c |
| Fluorescent tube | In vitro bulblets (3 months old) |
0.91 ± 0.97 b | ND | ND | 2.98 ± 1.09 a | 3.90 ± 1.51 bc |
| Natural habitat | Commercial bulbs (wild type, 3 years old) | 68.4 ± 7.8 a | 0.6 ± 0.4 a | ND | ND | 69.0 ± 7.4 a |
* ND: Not detected. ** Mean ± standard error. Means within each column followed by the same letter(s) are not significantly different at 5% level by Fisher’s protected LSD test.