Table 2.
Minimum inhibitory concentrations (MIC) in (µg/mL) and mean diameter of inhibition zones (DIZ) in (mm) of C. indicum and C. morifolium essential oils against different pathogens determined by microdilution and agar diffusion method.
| Microorganisms | C. indicum | C. morifolium | Positive Control * | |||
|---|---|---|---|---|---|---|
| DIZ (mm) |
MIC (µg/mL) |
DIZ (mm) |
MIC (µg/mL) |
DIZ (mm) |
MIC (µg/mL) |
|
| Gram Positive Bacteria | ||||||
| Bacillus subtilis ATCC 6051 | 20.0 ± 0.2 | 62.5 | 15.0 ± 0.7 | 125 | 31.6 ± 0.3 | 0.08 |
| Staphylococcus aureus ATCC 29213 | 20.4 ± 0.5 | 250 | 19.5 ± 0.3 | 250 | 30.1 ± 0.6 | 0.16 |
| Staphylococcus capitis ATCC 35661 | 19.9 ± 0.4 | 125 | 16.1 ± 0.5 | 250 | 29.2 ± 0.1 | 0.08 |
| Staphylococcus epidermidis ATCC 14990 | 19.6 ± 0.7 | 250 | 17.4 ± 0.8 | 250 | 26.4 ± 0.2 | 0.16 |
| Streptococcus agalactiae ATCC 27956 | 19.2 ± 0.8 | 62.5 | 14.4 ± 0.6 | 125 | 30.1 ± 0.1 | 0.08 |
| Streptococcus pyogenes ATCC 12344 | 21.9 ± 0.9 | 62.5 | 17.5 ± 0.3 | 125 | 29.7 ± 0.0 | 0.08 |
| Gram Negative Bacteria | ||||||
| Escherichia coli ATCC 25922 | 13.8 ± 0.2 | >500 | 13.7 ± 0.8 | 500 | 32.1 ± 0.0 | 0.06 |
| Pseudomonas fluorescens ATCC 13525 | 12.3 ± 0.1 | >500 | 11.7 ± 0.1 | >500 | 27.3 ± 0.1 | 0.24 |
| Salmonella typhimurium ATCC 14028 | 12.1 ± 0.5 | >500 | 11.5 ± 0.1 | >500 | 24.6 ± 0.2 | 0.96 |
| Shigella flexneri ATCC 700930 | 11.2 ± 0.9 | >500 | 10.9 ± 0.5 | >500 | 21.2 ± 0.0 | 0.96 |
| Fungi | ||||||
| Aspergillus fumigatus ATCC 1022 | 15.8 ± 0.5 | >500 | 14.5 ± 0.5 | 500 | 26.3 ± 0.1 | 0.12 |
| Candida albicans ATCC 90028 | 13.6 ± 0.7 | >500 | 12.6 ± 0.2 | >500 | 24.8 ± 0.7 | 0.24 |
| Geotrichum candidum ATCC 12784 | 14.0 ± 0.5 | 500 | 13.3 ± 0.4 | >500 | 23.2 ± 0.3 | 0.48 |
| Syncephalastrum racemosum ATCC 14831 | 11.9 ± 0.6 | >500 | 11.2 ± 0.7 | >500 | 21.4 ± 0.5 | 0.48 |
Data are presented as means ± S.D. n = 3; * The positive control was taken as ampicillin for Gram-positive bacteria, gentamycin for Gram-negative bacteria and clotrimazole for fungi; all assays consisted of 30 mg essential oil in 1 mL DMSO, and 100 μL were applied. DIZ, diameter of inhibition zone is measured in (mm) by the agar diffusion method.