Table 1.
Sequence of primers/probes used in PCR.
| Target Gene | Accession No. | Primer and Probe | Sequence (5′–>3′) | Length | Size (bp) | |
|---|---|---|---|---|---|---|
|
Aggregatibacter
actinomycetemcomitans |
16S ribosomal RNA | M75039.1 | Forward primer | CAAGTGTGATTAGGTAGTTGGTGGG | 25 | 220 |
| Reverse primer | CCTTCCTCATCACCGAAAGAA | 21 | ||||
| Probe | ATCGCTAGCTGGTCTGAGAGGATGGCC | 27 | ||||
|
Porphyromonas
gingivalis |
FimA type II (fimA) | KF770042.1 | Forward primer | GGAGTCTAATCTATTCGGTGCTTC | 24 | 132 |
| Reverse primer | GTGTAGTCTCTTCCCAACCAG | 21 | ||||
| Probe | ATTTCAACGGTGCTTATTCCCCTGC | 25 | ||||
|
Tannerella
forsythia |
karilysin protease gene | GQ856797 | Forward primer | TGGAGAATCAGTAACGGTTGG | 21 | 149 |
| Reverse primer | CCCCAACCACATTCACTACG | 20 | ||||
| Probe | TCCATTAAGCCCATTGCCCGGAA | 23 | ||||
|
Treponema
denticola |
OpdB gene | AF355459 | Forward primer | TCCGAGTGTTTACAGCCTTG | 20 | 94 |
| Reverse primer | GTCCTCATACCACTTTTCTCCC | 22 | ||||
| Probe | CTTCCGCCCCTGATTTGAGCAAC | 23 | ||||
|
Fusobacterium
nucleatum |
16S ribosomal RNA gene | FJ471640 | Forward primer | CGATAAGTAATCCGCCTGGG | 20 | 137 |
| Reverse primer | TCCTAAGATGTCAAACGCTGG | 21 | ||||
| Probe | CGTCGAATTAAACCACATGCTCCACC | 26 | ||||
|
Prevotella
intermedia |
hemagglutinin (phg) gene | AF017417 | Forward primer | ACATTGGAACTGAGACACGG | 20 | 145 |
| Reverse primer | GCCTCACTTTACTCCCCAAC | 20 | ||||
| Probe | TCAATCCTGCACGCTACTTGGCT | 25 | ||||
|
Prevotella
nigrescens |
gyrase subunit B (gyrB) gene | KF186729 | Forward primer | AGAATACGAACAGGGAAAGCC | 21 | 136 |
| Reverse primer | ACGTGCAACTATATCCCACTG | 21 | ||||
| Probe | TTGGTGAAACCGATAAGACTGGTACGC | 29 | ||||
|
Parvimonas
micra |
16S ribosomal RNA gene | AF542231 | Forward primer | ATGAATGCTAGGTGTTGGGAG | 21 | 141 |
| Reverse primer | GAATTAAACCACATGCTCCGC | 23 | ||||
| Probe | AGTTTCAGTGTTGCCACCGTACTCC | 25 | ||||
|
Campylobacter
rectus |
groEL gene | AB071388 | Forward primer | GCTACGGAGACTGAGATGAAAG | 22 | 136 |
| Reverse primer | TTAGATCCACTTTCGCACCG | 20 | ||||
| Probe | ATGCCTTCTTCTACAGCCGCCTT | 23 | ||||
|
Eubacterium
nodatum |
16S ribosomal RNA gene | U13041.1 | Forward primer | TCGTAAACTTCTGTCCAAAGGG | 22 | 138 |
| Reverse primer | CACCTACATACTCTTTACGCCC | 22 | ||||
| Probe | TAATTCCGGATAACGCTCGCCCC | 23 | ||||
|
Eikenella
corrodens |
proline iminopeptidase (pip) | AY198131 | Forward primer | CCTAACGATATGCCTGGAACC | 21 | 149 |
| Reverse primer | TCGATACTCCGTTTGCCATC | 20 | ||||
| Probe | AGTTTGCCGCCTAGTTTCATCCCT | 24 | ||||
|
Streptococcus
mutans |
PTS EII (mtlA) | AF210133 | Forward primer | CCTTCCTAGTCGCTTCCATTATC | 23 | 112 |
| Reverse primer | ACTGCTTGACCTTTAGACTCG | 23 | ||||
| Probe | TTTTGCTGCTTGTGTCACTGCTTGT | 27 | ||||
|
Staphylococcus
aureus |
translation elongation factor Tu (tuf) gene | AF298796.1 | Forward primer | CTTCCCAGGTGACGATGTAC | 22 | 132 |
| Reverse primer | TCACGTTCTGGAGTTGGAATG | 23 | ||||
| Probe | AGCTTTAGAAGGCGATGCTCAATACGAA | 28 | ||||
|
Enterococcus
faecalis |
16S ribosomal RNA gene | EU887827.1 | Forward primer | AACTGTTCATCCCTTGACGG | 22 | 137 |
| Reverse primer | TCAGACTTAAGAAACCGCCTG | 21 | ||||
| Probe | ACGCTTGCCACCTACGTATTACCG | 24 | ||||
|
Actinomyces
viscosus |
16S rRNA gene | X82453.1 | Forward primer | GGGAGCGAACAGGATTAGATAC | 24 | 148 |
| Reverse primer | CCTTTGAGTTTTAGCCTTGCG | 23 | ||||
| Probe | ACACCTAGTGCCCAACGTTTACGG | 24 | ||||
| GAPDH | Human ORF | LT737735.1 | Forward primer | GAAGGTGAAGGTCGGAGTC | 19 | 220 |
| Reverse primer | GAAGATGGTGATGGGATTTC | 20 | ||||
| Probe | CAAGCTTCCCGTTCTCAGCC | 20 | ||||
| beta-actin | Homo sapiens actin beta (ACTB), mRNA | NM_001101.4 | Forward primer | AGTCCCTTGCCATCCTAAAAG | 22 | 92 |
| Reverse primer | CAATGCTATCACCTCCCCTG | 21 | ||||
| Probe | CCAGTCCTCTCCCAAGTCCACAC | 24 |