Table 6.
Oligonucleotide primers for multiplex PCR detection of C. perfringens toxin genes.
| Toxin Gene | Primers | Primer Sequence (5′–3′) | Product Size (bp) |
|---|---|---|---|
| cpa (α-toxin) | CPA F | GCTAATGTTACTGCCGTTGA | 324 bp |
| CPA R | CCTCTGATACATCGTGTAAG | ||
| cpb (β-toxin) | CPB F | GCGAATATGCTGAATCATCTA | 195 bp |
| CPB R | GCAGGAACATTAGTATATCTTC | ||
| cpb2 (β2-toxin) | CPB2 F | AAATATGATCCTAACCAACAA | 548 bp |
| CPB2 R | CCAAATACTCTAATCGATGC | ||
| etx (ε-toxin) | ETX F | TGGGAACTTCGATACAAGCA | 376 bp |
| ETX R | AACTGCACTATAATTTCCTTTTCC | ||
| iap (ι-toxin) | IA F | AATGGTCCTTTAAATAATCC | 272 bp |
| IA R | TTAGCAAATGCACTCATATT | ||
| cpe (enterotoxin) | CPE F | TTCAGTTGGATTTACTTCTG | 485 bp |
| CPE R | TGTCCAGTAGCTGTAATTGT |