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. 2020 Oct 14;10(10):1440. doi: 10.3390/biom10101440

Figure 2.

Figure 2

Erinacine C induced neurotrophic activity mediates PC12 differentiation via MAPK/ERK, PLCγ and PI3K signaling. (A) Schematic drawing of signaling pathways downstream of the NGF-activated TrkA receptor and respective pharmacological inhibitors. (B) Percentage of PC12 cells differentiated into neuron-like cells upon stimulation with either recombinant NGF (left) or conditioned medium of erinacine C treated 1321N1 astrocytoma cells (right) in the presence of signal transduction cascade inhibitors (two independent fields, n = 100 cells; three independent cultures). (C) In the same cultures individual neurite length of differentiated cells were measured and are displayed as average values (n = 180 cells, three independent cultures) as an indication of the extent of differentiation. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.