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. 2020 Oct 14;10(10):1440. doi: 10.3390/biom10101440

Figure 5.

Figure 5

Erinacine C activates ETS-reporter construct in a concentration dependent manner. (A) Series of constructed reporter plasmids containing 2, 4, 6, or 8 ETS1 binding sites followed by the E1b minimal promoter and Firefly luciferase. (B) Transfection of these constructs into 1321N1 cells followed by incubation with either 3 or 5 µg/mL of erinacine C for 24 h. Fold-upregulation of luciferase activity in dependence of the different erinacine C concentrations and increasing numbers of ETS binding sites are displayed. (C) Fold-upregulation of luciferase activity with the 8 x ETS reporter construct upon increasing erinacine C concentrations up to 5 µg/mL. Higher erinacine C concentrations yielded lower luciferase activity values probably due to altered expression responses in 1321N1 cells. (D) Fold-upregulation of luciferase activity with the 8 x ETS reporter construct and 5 µg/mL erinacine C incubation in dependence of varying incubation times. EtOH treated cells served as controls for which luciferase activity was set as one in all displayed assays (red line) (four values per treatment, four independent cultures). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.