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. 2020 Oct 14;10(10):1440. doi: 10.3390/biom10101440

Figure 7.

Figure 7

Erinacine C likely activates ETS signaling independent of inducing neurotrophin expression. (A) Fold-activation of ngf (dark grey) and bdnf (light grey) transcription in 1321N1 cells treated for 48 h with erinacine C in the presence of MAPKK (20 µM PD98059) and ERK1/2 (10 nM U0126) inhibitors compared to gapdh mRNA analyzed by semiquantitative RT-PCR. Values from 0.5% EtOH treated cells were used as control and were set as one (red line). Stars indicate a significant upregulation compared to EtOH controls. (B) Fold-activation of ngf (dark grey) and bdnf (light grey) expression by semiquantitative RT-PCR from 1321N1 cells electroporated either with the pBluescriptII vector (vehicle) or the pCS-ETS1DBD-KRAB construct (KRAB) expressing a constitutive inhibitor of ETS signaling. A total of 24 h after transfection cells were exposed to erinacine C (5 µg/mL) or EtOH (0.5%) for 48 h. The latter served as control with its ngf:gapdh ratio set as one (marked as red line) (three independent cultures). ns: not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.