Table 1.
List of the identified peptides discovered in SCX-S2, RP-R7, and RP-R13 fractions.
| No | Protein | Peptide | m/z | Retention Time |
|---|---|---|---|---|
| Identified peptides in fraction S2 from off-line SCX | ||||
| 1 | Vitellogenin-1-like (gi: 558222264) | VPGLAL | 569.36 | 30.31 |
| 2 | Vitellogenin-1-like (gi: 558222264) | LPSW | 502.27 | 29.00 |
| 3 | Vitellogenin-2-like (gi: 1394701588) | LPLF | 489.31 | 32.31 |
| 4 | Tesmin isoform X4 (gi: 1394748730) | LVGLPL | 611.41 | 35.30 |
| 5 | Low quality protein: M-phase phosphoprotein 9 (gi: 1394664164) | WLQL | 559.32 | 33.05 |
| 6 | Vitellogenin-2-like (gi: 1394701588) | VLPSENPVFK | 565.31 | 26.29 |
| 7 | Vitellogenin-2-like (gi: 1394701588) | YSLY | 542.26 | 28.84 |
| 8 | Vitellogenin-1-like (gi: 558222264) | LAAF | 421.24 | 24.73 |
| 9 | Vitellogenin-1-like (gi: 558222264) | NAPLY | 577.29 | 25.50 |
| 10 | Apolipoprotein B-100 (gi: 946679552) | LNEYLEDLR | 582.79 | 33.85 |
| 11 | Apolipoprotein B-100 (gi: 946679552) | LLLY | 521.33 | 32.25 |
| 12 | Apolipoprotein B-100 (gi: 946679552) | LGLL | 415.29 | 30.31 |
| 13 | Vitellogenin-2-like (gi: 1394701588) | PISLPVGPPVPESA | 680.38 | 36.08 |
| 14 | Vitellogenin-1-like (gi: 558222264) | AQISPAPSSDF | 1119.53 | 27.69 |
| 15 | Vitellogenin-1-like (gi: 558222264) | ILDIMPAVSK | 543.81 | 30.05 |
| 16 | Vitellogenin-1-like (gi: 558222264) | MPGYAPSASDL | 1108 | 30.00 |
| 17 | Vitellogenin-1-like (gi: 558222264) | ISPAPSSDF | 920.43 | 26.41 |
| 18 | Vitellogenin-1-like (gi: 558222264) | SDDGLNF | 767.32 | 30.39 |
| 19 | Vitellogenin-2-like (gi: 1394701588) | YQIGAIE | 793.41 | 27.97 |
| 20 | Vitellogenin-1-like (gi: 558222264) | SGVGTQW | 734.35 | 25.17 |
| 21 | Vitellogenin-1-like (gi: 558222264) | ILDMPA | 772.42 | 34.53 |
| Identified peptides in fraction R7 from RP-HPLC | ||||
| 1 | Vitellogenin-1-like (gi: 558222264) | VPGLAL | 569.36 | 30.54 |
| 2 | Vitellogenin-1-like (gi: 558222264) | LPSW | 502.26 | 29.16 |
| 3 | Vitellogenin-1-like (gi: 558222264) | QELVQELHQF | 635.82 | 27.73 |
| 4 | Vitellogenin-2-like (gi: 1394701588) | SVPPELHL | 446.25 | 26.67 |
| 5 | Vitellogenin-1-like (gi: 558222264) | IRNAPLY | 423.74 | 20.08 |
| 6 | Apolipoprotein B-100 (gi: 946679552) | KIPEVTL | 400.25 | 26.05 |
| 7 | Vitellogenin-2-like (gi: 1394701588) | FADHPAIQ | 449.72 | 20.16 |
| Identified peptides in fraction R13 from RP-HPLC | ||||
| 1 | Vitellogenin-2-like (gi: 1394701588) | LPLF | 489.31 | 33.58 |
| 2 | Tesmin isoform X4 (gi:1394748730) | LVGLPL | 611.41 | 35.50 |
| 3 | Low quality protein: M-phase phosphoprotein 9 (gi:1394664164) | WLQL | 559.32 | 33.40 |
| 4 | Vitellogenin-2-like (gi: 1394701588) | LVGL | 401.27 | 34.82 |
| 5 | Vitellogenin-1-like (gi: 558222264) | PPGL | 383.23 | 30.30 |
| 6 | Vitellogenin-1-like (gi: 558222264) | QVESQLVENLR | 657.85 | 31.63 |
| 7 | Vitellogenin-1-like (gi: 558222264) | YLGDVLPGLPR | 600.34 | 34.18 |
| 8 | Pepsin A-like, partial (gi: 1394653157) | GLLGL | 472.31 | 34.34 |
| 9 | Apolipoprotein B-100 (gi: 946679552) | FLDLVIK | 424.26 | 32.17 |