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. 2020 Oct 30;39:230. doi: 10.1186/s13046-020-01743-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Lycorine hydrochloride induces apoptosis of BCL2-drug-resistant gastric cancer cell lines. a Screening of HA14–1 resistant cell lines (Detailed screening methods was described in the materials and methods). b Western blotting analysis of MCL1 expression in MKN-45-R and SGC-7901-R cell lines expressing shGFP, shMCL1#2, shMCL1#4. Trypan blue staining was used to analyze the apoptosis induced by DMSO or HA14–1 in MKN-45-R and SGC-7901-R cell lines expressing shGFP, shMCL1#1. c, d Apoptosis was analyzed in MKN-45-R, SGC-7901-R cells after treatment with 20 μM LH for 48 h by trypan blue and TUNEL staining. Apoptotic rate of MKN-45-R, SGC-7901-R cells in panel was quantified. e, f The expression of apoptotic protein, including C-Caspase 9, C-Caspase 3, PARP and C-PARP in BCL2-drug-resistant gastric cancer cells treated with LH at different concentrations and time gradients. DMSO was used as control. Tubulin was used as internal reference. All data were analyzed by unpaired Student’s t-tests and were showed as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001