Table 1.
Ingredients and proximate composition of Hermetia illucens larva meal and experimental diets.
| Ingredients (g/kg) | H. illucens Larva Meal | CON | H20 | H40 | H60 |
|---|---|---|---|---|---|
| FM (Chile, super prime) a | - | 720 | 570 | 420 | 270 |
| H. illucens larva meal b | 0 | 200 | 400 | 600 | |
| Wheat meal | - | 120 | 90 | 60 | 30 |
| Fish oil | - | 60 | 40 | 20 | 0 |
| Starch, D500 | - | 80 | 80 | 80 | 80 |
| Mineral mixture c | - | 10 | 10 | 10 | 10 |
| Vitamin mixture d | - | 10 | 10 | 10 | 10 |
| Proximate compositione | |||||
| DM (g/100 g) | 94.18 | 88.74 | 90.76 | 90.59 | 90.51 |
| CP (g/100 g DM) | 55.34 | 54.50 | 54.37 | 54.10 | 53.91 |
| EE (g/100 g DM) | 17.97 | 11.92 | 11.95 | 11.62 | 11.64 |
| Ash (g/100 g DM) | 7.12 | 14.77 | 13.70 | 12.44 | 11.41 |
| Chitin (g/100 g DM) | 5.00 | - | 0.98 | 2.12 | 3.15 |
| NFE (g/100 g DM) f | 14.57 | 18.81 | 19.02 | 19.72 | 19.89 |
| Gross energy (MJ/kg DM) g | 22.90 | 22.54 | 23.02 | 23.26 |
FM, fishmeal; DM, dry matter; CP, crude protein; EE, ether extract; NFE, nitrogen free extracts, groups CON, H20, H40 and H60 represent 0%, 20%, 40% and 60% inclusion of H. illucens meal, respectively. a Fishmeal was purchased from Corpesca S.A. (Santiago, Chile). Proximate composition (% as-fed basis): 90.4 DM; 66.7 CP; 8.3 EE; 14.9 Ash. b Hermetia illucens larvae meal purchased from Hermetia Deutschland GmbH & Co. KG (Baruth/Mark, Germany). c Mineral mixture (g or mg/kg diet): bicalcium phosphate 500 g, calcium carbonate 215 g, sodium salt 40 g, potassium chloride 90 g, magnesium chloride 124 g, magnesium carbonate 124 g, iron sulphate 20 g, zinc sulphate 4 g, copper sulphate 3 g, potassium iodide 4 mg, cobalt sulphate 20 mg, manganese sulphate 3 g, sodium fluoride 1 g (Granda Zootecnica, Cuneo, Italy). d Vitamin mixture (IU or mg/kg diet): DL-tocopherolacetate, 60 IU; sodium menadione bisulphate, 5 mg; retinylacetate, 15,000 IU; DL-cholecalciferol, 3000 IU; thiamine, 15 mg; riboflavin, 30 mg; pyridoxine, 15 mg; vitamin B12, 0.05 mg; nicotinic acid, 175 mg; folic acid, 500 mg; inositol, 1000 mg; biotin, 2.5 mg; calcium panthotenate, 50 mg; choline chloride, 2000 mg (Granda Zootecnica, Cuneo, Italy). e Values are reported as mean of triplicate analyses. f Calculated as 100 − (CP + EE + Ash + Chitin). g Determined by bomb calorimetry.